Journal: Journal of Cellular and Molecular Medicine

Article Title: The α2AR/Caveolin‐1/p38MAPK/NF‐κB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia‐reperfusion

doi: 10.1111/jcmm.16614

Figure Lengend Snippet: Dex reduces lung injury following intestinal I/R via Cav‐1‐dependent p38MAPK/NF‐κB pathway inactivation. A, Comparison of the down‐regulation for Cav‐1 protein using three anti‐Cav‐1 shRNA constructs. * P < .05 compared with the mock group. Pre‐injections were applied four days before intestinal I/R injury. B, RT‐qPCR of Cav‐1 mRNA expression and Western blot analysis of Cav‐1, p38, p‐p38, p‐p65 and p65 expression in rat lung tissues with Cav‐1 overexpression or knockdown before intestinal I/R injury. C, ELISA detection of TNF‐α expression in rat lung tissues with Cav‐1 overexpression or knockdown before intestinal I/R injury. D, ELISA detection of IL‐1β expression in rat lung tissues with Cav‐1 overexpression or knockdown before intestinal I/R injury. E, ELISA detection of IL‐6 expression in rat lung tissues with Cav‐1 overexpression or knockdown before intestinal I/R injury. F, Lung injury score of rat lung tissues with Cav‐1 overexpression or knockdown before intestinal I/R injury. G, Apoptosis rate of rat lung tissues with Cav‐1 overexpression or knockdown before intestinal I/R injury. * P < .05 vs sham group, # P < .05 vs I/R + oe‐NC group. H, Western blot analysis of Cav‐1, p38, p‐p38, p‐p65 and p65 in rat lung tissues upon Dex treatment and/or Cav‐1 knockdown prior to intestinal I/R injury. I, ELISA detection of TNF‐α expression in rat lung tissues upon Dex treatment and/or Cav‐1 knockdown prior to intestinal I/R injury. J, ELISA detection of IL‐1βexpression in rat lung tissues upon Dex treatment and/or Cav‐1 knockdown prior to intestinal I/R injury. K, ELISA detection of IL‐6 expression in rat lung tissues upon Dex treatment and/or Cav‐1 knockdown prior to intestinal I/R injury. L, Lung injury score of rat lung tissues upon Dex treatment and/or Cav‐1 knockdown prior to intestinal I/R injury. M, Apoptosis rate of rat lung tissues upon Dex treatment and/or Cav‐1 knockdown prior to intestinal I/R injury, detected by TUNEL staining. * P < .05 vs sham group, # P < .05 vs I/R + Dex +sh‐NC group. Data among multiple groups were analysed by one‐way ANOVA with Tukey's post hoc test

Article Snippet: The PVDF membrane was blocked in 5% skimmed milk at room temperature for 1 hours and incubated overnight at 4°C with the diluted primary antibodies: rabbit anti‐α 2A ‐AR (1:1000, 14266‐1‐AP, Proteintech ProteinTech Group), rabbit anti‐Cav‐1 (1:1000, ab32577, Abcam Inc.), rabbit anti‐p38 (1:1000, ab31828, Abcam), rabbit anti‐phosphorylated (p)‐p38 (1:1000, ab4822, Abcam), rabbit anti‐NF‐κBp65 (1:1000, ab16502, Abcam), rabbit anti‐NF‐κB‐p‐p65 (1:1000, ab86299, Abcam) and rabbit anti‐Actin (1:1000, ab179467, Abcam).

Techniques: Comparison, shRNA, Construct, Quantitative RT-PCR, Expressing, Western Blot, Over Expression, Knockdown, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Staining

Journal: The Journal of Veterinary Medical Science

Article Title: Inhibition of transient receptor potential vanilloid type 1 through α 2 adrenergic receptors at peripheral nerve terminals relieves pain

doi: 10.1292/jvms.21-0429

Figure Lengend Snippet: Sequences of primers for RT-PCR

Article Snippet: Subsequently, the sections were incubated with a guinea pig anti-TRPV1 antibody (Merck Millipore corporation, Temecula, CA, USA, Cat# AB5566, Lot# 3135959) diluted to 1:50, a rabbit anti-α 2A antibody (Cusabio Technology, Houston, TX, USA, Cat# CSB-PA007424, Lot# G1229Y) diluted to 1:100 or a rabbit anti-α 2C antibody (Cusabio Technology, Cat# CSB-PA000916, Lot# G1229Y) diluted to 1:100 overnight at 4°C.

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: Inhibition of transient receptor potential vanilloid type 1 through α 2 adrenergic receptors at peripheral nerve terminals relieves pain

doi: 10.1292/jvms.21-0429

Figure Lengend Snippet: Subtypes of the α 2 adrenergic receptors detected in dorsal root ganglia. A. The real-time PCR amplification curves are shown in the left panel. A threshold was set for 3, and the Ct values of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α 2 receptors were obtained. The ΔCt values of α 2A (n=8), α 2B (n=8) and α 2C (n=8) receptors against GAPDH were calculated and are shown in the right panel. The columns represent the mean ± the standard error of the mean. B, C. Images of the immunoreactivities of TRPV1, α 2A and α 2C receptors in rat dorsal root ganglia. The TRPV1-, α 2A receptor- and α 2C receptor-like immunoreactivities were visualized by 3,3′-diaminobenzidine. Arrows, positively stained small-sized neurons; arrowheads, positively stained medium-sized neurons.

Article Snippet: Subsequently, the sections were incubated with a guinea pig anti-TRPV1 antibody (Merck Millipore corporation, Temecula, CA, USA, Cat# AB5566, Lot# 3135959) diluted to 1:50, a rabbit anti-α 2A antibody (Cusabio Technology, Houston, TX, USA, Cat# CSB-PA007424, Lot# G1229Y) diluted to 1:100 or a rabbit anti-α 2C antibody (Cusabio Technology, Cat# CSB-PA000916, Lot# G1229Y) diluted to 1:100 overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Staining